a2ar antagonist sch442416 Search Results


a2ar antagonists  (Tocris)
94
Tocris a2ar antagonists
A2ar Antagonists, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(+/-)-5'-chloro-5'-deoxy-enba  (Bio-Techne corporation)
90
Bio-Techne corporation (+/-)-5'-chloro-5'-deoxy-enba
(+/ ) 5' Chloro 5' Deoxy Enba, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a2ar antagonist sch442416  (Tocris)
93
Tocris a2ar antagonist sch442416
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
A2ar Antagonist Sch442416, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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zm 241385  (Tocris)
90
Tocris zm 241385
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Zm 241385, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sch 442416 2-(2-furanyl)7-[3-(4-methoxyphenyl)propyl]-7h-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine  (Millipore)
90
Millipore sch 442416 2-(2-furanyl)7-[3-(4-methoxyphenyl)propyl]-7h-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Sch 442416 2 (2 Furanyl)7 [3 (4 Methoxyphenyl)Propyl] 7h Pyrazolo[4,3 E][1,2,4]Triazolo[1,5 C]Pyrimidin 5 Amine, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sch 442416 2-(2-furanyl)7-[3-(4-methoxyphenyl)propyl]-7h-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine - by Bioz Stars, 2026-03
90/100 stars
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regadenoson  (Astellas)
90
Astellas regadenoson
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Regadenoson, supplied by Astellas, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cocaine hydrochloride  (Millipore)
90
Millipore cocaine hydrochloride
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Cocaine Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sprague-dawley rats  (Dawley Inc)
90
Dawley Inc sprague-dawley rats
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Sprague Dawley Rats, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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istradefylline kw 6002 8-[(e)-2-(3,4-dimethoxyphenyl)etheyl]-1,3diethyl-7-methylpurine-2,6-dione  (Millipore)
90
Millipore istradefylline kw 6002 8-[(e)-2-(3,4-dimethoxyphenyl)etheyl]-1,3diethyl-7-methylpurine-2,6-dione
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Istradefylline Kw 6002 8 [(E) 2 (3,4 Dimethoxyphenyl)Etheyl] 1,3diethyl 7 Methylpurine 2,6 Dione, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrs1706 (n-(4-acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1hpurin-8-yl)penoxy]-acetamide  (Tocris)
90
Tocris mrs1706 (n-(4-acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1hpurin-8-yl)penoxy]-acetamide
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Mrs1706 (N (4 Acetylphenyl) 2 [4 (2,3,6,7 Tetrahydro 2,6 Dioxo 1,3 Dipropyl 1hpurin 8 Yl)Penoxy] Acetamide, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mrs1706 (n-(4-acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1hpurin-8-yl)penoxy]-acetamide - by Bioz Stars, 2026-03
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sch442416 (2-(2-furanyl)-7-[3-(4-methoxyphenyl)propyl]-7h-pyrazolo[4, 3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine  (Tocris)
90
Tocris sch442416 (2-(2-furanyl)-7-[3-(4-methoxyphenyl)propyl]-7h-pyrazolo[4, 3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Sch442416 (2 (2 Furanyl) 7 [3 (4 Methoxyphenyl)Propyl] 7h Pyrazolo[4, 3 E][1,2,4]Triazolo[1,5 C]Pyrimidin 5 Amine, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sch442416 (2-(2-furanyl)-7-[3-(4-methoxyphenyl)propyl]-7h-pyrazolo[4, 3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine - by Bioz Stars, 2026-03
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ccpa (2-chloro-n6-cyclpentyladenosine  (Tocris)
90
Tocris ccpa (2-chloro-n6-cyclpentyladenosine
( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the <t>A2AR</t> agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .
Ccpa (2 Chloro N6 Cyclpentyladenosine, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the A2AR agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .

Journal: JCI Insight

Article Title: IL-4 prevents adenosine-mediated immunoregulation by inhibiting CD39 expression

doi: 10.1172/jci.insight.157509

Figure Lengend Snippet: ( A and B ) T cells were stimulated in the absence or presence of the STAT6 inhibitor AS1517499 (100 nM for A ) or 20 ng/mL IL-4 for B. Cells were resuspended in medium supplemented with ATP at 20 μM on day 4 and eATP concentrations were determined after 30 minutes and 1 hour. Results are shown as a percent decline from original concentration. ( C ) Purified T cells were activated for 4 days in the absence or presence of AS1517499, then restimulated with newly added Dynabeads for 6 hours before cells were collected for intracellular cytokine staining and flow cytometry analysis. ( D – F ) Day 4-activated T cells were restimulated with newly added Dynabeads plus/minus adenosine (500 μM for D ), or the A2AR agonist CGS21680 (100 nM for E), or the P2RX antagonist oATP (100 μM for F) for 6 hours and then stained for intracellular cytokines. ( G ) Purified T cells were treated with STAT6 inhibitor AS1517499, A2AR antagonist SCH58621, or both during T cell activation by Dynabeads. On day 4, cells were restimulated by PMA/IONO for 4 hours before being collected for intracellular cytokine staining. Results are pooled from 3 experiments. Data in C are shown as mean ± SEM and compared by paired 2-tailed Student’s t test in A – G .

Article Snippet: Cells were activated for 4 days by anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific, catalog 11132D; bead to cell ratio 1:2) in the presence or absence of cytokines or chemical compounds, including STAT6 inhibitor AS1517499 (100 nM; Axon Medchem, catalog Axon 1992), IL-4 (20 ng/mL; Peprotech, catalog 200-04), and A2AR antagonist SCH442416 (10 μM; TOCRIS, catalog 2463).

Techniques: Concentration Assay, Purification, Staining, Flow Cytometry, Activation Assay